Supplementary Figure 2: Limiting dilution transplantation to assess fidelity of Confetti-based estimates, efficiency of Confetti labeling in adult PB of +/Cre mice and Log10(CV)s of Confetti frequency in AGM explant recipient PB.
From: Lifelong haematopoiesis is established by hundreds of precursors throughout mammalian ontogeny
(a) 5 × 106-2 × 104CD45.2 + ROSA26+/ConfettiFlk1+/Cre WBM was transplanted at limiting dilution into irradiated CD45.1 + CD45.2 + mice along with 2 × 105 CD45.1 + WBM cells (see also Fig. 2c). Total CD45.2 + PB chimerism 16 weeks post-transplant is indicated. Black dots represent individual engrafted recipients and red dots represent individual non-engrafted recipients. (b) LDA was applied to estimate number of repopulating units (RUs). The data fit well the LDA assumption (Pearson Chi-square and Deviance Chi-square >0.05). (c) Average Confetti labeling efficiency in adult PB of ROSA26+/Confetti + /Cre mice. ROSA26+/ConfettiE2a+/Cre (E2aCre, n = 13 mice), ROSA26+/ConfettiFlk1+/Cre (Flk1Cre, n = 7), ROSA26+/ConfettiVE-Cadherin+/Cre (VE-CadherinCre, n = 12), ROSA26+/ConfettiVav1+/Cre (Vav1Cre, n = 10), ROSA26+/ConfettiUbiquitin+/ERT2-Cre (UbiquitinERT2-Cre) treated at E7.5 (n = 5) or E8.5 (n = 6) and ROSA26+/Confetti Cdh5+/ERT2-Cre (Cdh5ERT2-Cre) treated at E8.5 (n = 5) or E9.5 (n = 7). Error bars indicate ±s.d. of mean. Individual data points are shown in red. (d) Log10(CV) of sample-to-sample variance in Confetti color distribution in CD45.2 + PB of recipients of 1.0EE AGM explant-derived cells at 16 weeks post-transplant. Variance calculated based on 7 mice. Source data are provided in Supplementary Table 1.
