Supplementary Figure 6: Inhibition of miR25/93 or cGAS is unable to affect cytoplasmic level of mtDNA as a response to hypoxia.
From: miR-25/93 mediates hypoxia-induced immunosuppression by repressing cGAS
(a) qPCR analysis for cytosolic mtDNA level in MCF7 or MDA-MB-231stable transfectants expressing shRNA against miR25/93 vs. control cells. (b) Knockdown of cGAS was unable to affect the hypoxic effect on increasing the level of cytosolic mtDNA. The cells expressing shRNA targeting EGFP were used as control. N: normoxia H: hypoxia. All the data are presented as means ± SD (n = 3 independent experiments). Samples were compared using two-tailed Student’s t test. Asterisk indicates P < 0.05, compared with controls.
