Supplementary Figure 2: miR-16 reduces TYRP1 mRNA decay induced by miR-155.

(a) Cartoon illustrating the 3′UTR of human TYRP1 with the position of the SNPs rs683 and rs910, the three MRE-155 sites (blue) and the three putative MRE-16 sites (orange). (b) MRE-155 sequences on TYRP1 3′UTR. TYRP1-C corresponds to the NM_000550.2 and TYRP1-A to NM_000550.1. Alignments have been performed using RNAhybrid³⁴. Underlined sequences on MRE-155#2C and MRE-155#3A are detailed in boxes on the right to show the position of the SNPs in the two alleles of TYRP1. Arrows indicate the SNP rs683 and rs910 positions. (c) Effects of synthetic miR-155 on the identified regions of TYRP1 MRE-155 in 501Mel. Luciferase activity was evaluated 48 h after transfection. Each histogram represents the mean ± s.d. of n = 3 biologically independent experiments; two-sided unpaired t-test with Welch’s correction; ^∗P < 0.05. (d) Northern blot quantification of miR-16 in 501Mel cells. The signal (from 501Mel cells) was fit to the standard curve from synthetic titration signals to give final copy number per cell. RNU6 served as a loading control. Pictures are representative of three experiments. (e) Northern blot quantification of TYRP1 mRNA in 501Mel cells. The signal (from 501Mel cells) was fit to the standard curve from the TYRP1 3′UTR’s synthetic titration signal to give final copy number per cell. GAPDH serves as a loading control. Picture presents three biological replicates of 501Mel. Source data are available in Supplementary Table 8.