Supplementary Figure 3: MRE-16 on human and mouse TYRP1 mRNA and biological consequences.

(a) MRE-16s’ sequences on mouse TYRP1 3′UTR (NM_031202.3). Alignments have been performed using RNAhybrid³⁴. (b) Schematic representation of the interaction (purple base paring) of miR-16 (orange) and miR-155 (blue) with human and mouse TYRP1 MRE-16#3 and MRE-155#3 respectively. (c,d) TYRP1 knockdown in mouse B16-F10 melanoma cells using three different siRNAs. TYRP1 mRNA levels (c) and cell density (d) have been respectively evaluated at 5 and 3 days after siRNA transfection; one-way ANOVA with Holm-Sidak’s multiple comparisons test. (e) Effect on cell density of synthetic miR-16 transfected in mouse B16-F10 melanoma cells 3 days after miRNA transfection; two-sided unpaired t-test with Welch’s correction, ^∗P < 0.05. Each histogram represents the mean ± s.d. (n = 3 biologically independent experiments). Source data are available in Supplementary Table 8.