Supplementary Figure 4: Characterization of MSCs and hematopoietic repopulating cells isolated from BM aspirates of healthy human donors or AML patients.

(a, b) Phase contrast images and FACS plots of MSCs isolated from the BM of healthy adult donors (a) and AML patients (b). Scale bars, 100 μm. (c) FACS analysis of human hematopoietic repopulation in the BM of mice transplanted with 10 × 10⁶ BM mononuclear cells from AML Patients #12 or #13. Plots are representative of n = 4 (Patient#12), and n = 2 (Patient#13) independent xenografted mice, with similar results. (d,e) Heatmap (d) and bar graphs (e) showing the frequency of mature BODIPY⁺ adipocytes differentiated from healthy human BM-MSCs that were cultured in different cocktails of adipogenesis-inducing agents (variable concentrations of dexamethasone, IBMX, and insulin, together with a constant concentration of 2% FBS across all conditions). Each cocktail was tested in the presence or absence of 10 ng ml⁻¹ IL-6 as a known inhibitor of adipogenesis⁵⁹. Dashed lines indicate the final cocktail selected, based on the greatest sensitivity to detect inhibitory effects of IL-6. (d) Each heatmap value represents the average of two replicate wells. (e) Only values from 500 μM IBMX conditions are plotted. Source data can be found in Supplementary Table 8.