Supplementary Figure 3: Osteoblasts and yellow marrow adipocytes persist in BM despite substantial levels of leukemic disease.

(a) Representative PLN immunofluorescence images of femur sections from AML-engrafted mice or non-transplanted controls, corresponding to high magnification images shown in Fig. 3e. Scale bar, 250 μm. (b,c) PLN quantification of absolute adipocyte numbers in tail vertebrae sections. N numbers (and data points) represent independent xenografted mice from 2 experiments (precise n values indicated in the figure). Data values for Patient #2 correspond to frequencies shown in Fig. 3g. (d) High magnification images of H&E-stained mouse femurs engrafted with healthy human hematopoietic cells or human AML (Patient #2). Chimerism levels are similar between healthy and AML grafts (>90% human chimerism). Yellow arrowheads indicate osteoblasts. Scale bar, 20 μm. (e) Immunofluorescence quantification of Osx + osteoblasts in femur sections from AML-engrafted mice (Patient #6) or healthy human HSPC-engrafted mice. n = 2 mice (healthy) and n = 4 mice (AML) from 1 experiment. This was reproduced in an additional experiment using an independent AML patient sample (Fig. 3k). (f–h) MicroCT quantification of mineralized bone; additional parameters corresponding to data shown in Fig. 3l–n (AML-xenografts from Patient #2). n = 3 biologically independent mice/group from 1 experiment. All data are means ± s.e.m. Statistical significance was assessed by Mann–Whitney U test (b) or unpaired t-tests (c,f–h). No statistical analyses were performed in e. Source data can be found in Supplementary Table 8.