Supplementary Figure 4: Gene expression levels of PEG-4MAL-encapsulated spheroids are comparable to those embedded in MatrigelTM.
From: Synthetic hydrogels for human intestinal organoid generation and colonic wound repair
RNA levels of pluripotency (OCT4), endoderm (FOXA2), and epithelial junction (ZO1, ECAD and CLDN2) genes, as quantified by RT-qPCR (mean ± SEM; n = 6 samples per group). Unpaired two-tailed t-test was used to identify statistical differences between matrix types (∗∗P < 0.01; ns, not significant). One experiment was performed. Source data are available in Supplementary Table 1. Primer sequences are provided in Supplementary Table 2.
