Supplementary Figure 2: PEG-4MAL hydrogel supports hiPSC-derived intestinal spheroid development into HIOs comparable to hESC-derived spheroids.
From: Synthetic hydrogels for human intestinal organoid generation and colonic wound repair
Transmitted light and fluorescence microscopy images of hiPSC-derived HIO generation within (a) 4.0% PEG-4MAL hydrogels, (b) MatrigelTM, or (c) 8.0% PEG-4MAL hydrogels. hiPSC-derived spheroid and HIO viability was assessed at different time-points after encapsulation. (d) Transmitted light microscopy images of hESC-derived HIO generation within 4.0% PEG-4MAL hydrogels. (a, c, d) These organoids were never encapsulated within MatrigelTM. Black arrows show epithelial budding. Bar, 500 μm. Three independent experiments were performed and data is presented for one of the experiments. Every experiment was performed with 12 gel samples per experimental group (PEG-4MAL, MatrigelTM).
