Figure 5: Structural requirements for interaction of Hip-1 and Hippi. | Nature Cell Biology

Figure 5: Structural requirements for interaction of Hip-1 and Hippi.

From: Recruitment and activation of caspase-8 by the Huntingtin-interacting protein Hip-1 and a novel partner Hippi

Figure 5

a, Hip-1 pDED interacts with Hippi pDED in yeast. Liquid-culture yeast two-hybrid assays with o-nitrophenyl-β-D-galactopyranoside (ONPG) as β-galactosidase substrate were carried out in Y190 yeast expressing the Gal4 DB–Hip-1 pDED fusion protein and the indicated Gal4 AD fusion proteins. The level of interaction between the two fusion proteins is expressed in units of β-galactosidase activity. A representative experiment (n = 3) is shown. Standard deviation (error bars) is calculated for each strain from triplicate samples. b, The helix-5 compatibility residue in the pDED of Hip-1 and Hippi determines their level of interaction. The same procedure as in a was used to measure the level of interaction between Hip-1 pDED and Hippi pDED fusion proteins carrying various residues at the critical position indicated in Fig. 1b. The top line under the histogram indicates the amino acid present in Hip-1 pDED and the lower line that present in Hippi pDED. A representative experiment (n = 4) is shown. Standard deviation (error bars) is calculated for each strain from triplicate samples.

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