Skip to main content

Thank you for visiting nature.com. You are using a browser version with limited support for CSS. To obtain the best experience, we recommend you use a more up to date browser (or turn off compatibility mode in Internet Explorer). In the meantime, to ensure continued support, we are displaying the site without styles and JavaScript.

  • News & Views
  • Published:

NMR Technology

The competitive world of RAS biology

Nature Chemical Biology volume 10, pages 173–174 (2014)Cite this article

Subjects

System-wide approaches are gaining popularity over traditional studies of individual molecular components. A new NMR approach unravels the interaction hierarchy of these different components by placing them in direct competition for the same target.

Access through your institution
Buy or subscribe

This is a preview of subscription content, access via your institution

Access options

Access through your institution

Buy this article

  • Purchase on SpringerLink
  • Instant access to full article PDF
Buy now

Prices may be subject to local taxes which are calculated during checkout

Figure 1: In systems composed of a receptor P and three ligand proteins with similarly high binding constants (A, B, C), pairwise competition NMR experiments permit recovery of the concentration of PA, PB and PC complexes as a function of ligand concentration.

References

  1. Gierasch, L.M. & Gershenson, A. Nat. Chem. Biol. 5, 774–777 (2009).

    Article  CAS  Google Scholar 

  2. Kitano, H. Science 295, 1662–1664 (2002).

    Article  CAS  Google Scholar 

  3. Papin, J.A., Hunter, T., Palsson, B.O. & Subramaniam, S. Nat. Rev. Mol. Cell Biol. 6, 99–111 (2005).

    Article  CAS  Google Scholar 

  4. Smith, M.J. & Ikura, M. Nat. Chem. Biol. 10, 223–230 (2014).

    Article  CAS  Google Scholar 

  5. Cavalli, A., Salvatella, X., Dobson, C.M. & Vendruscolo, M. Proc. Natl. Acad. Sci. USA 104, 9615–9620 (2007).

    Article  CAS  Google Scholar 

  6. Fragai, M., Luchinat, C., Parigi, G. & Ravera, E. Coord. Chem. Rev. 257, 2652–2667 (2013).

    Article  CAS  Google Scholar 

  7. Tzeng, S.-R. & Kalodimos, C.G. Nat. Chem. Biol. 9, 462–465 (2013).

    Article  CAS  Google Scholar 

  8. Banci, L. et al. Nat. Chem. Biol. 9, 297–299 (2013).

    Article  CAS  Google Scholar 

  9. Banci, L. et al. Nature 465, 645–648 (2010).

    Article  CAS  Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Giacomo Parigi and Enrico Ravera are at CERM and the Department of Chemistry 'Ugo Schiff', Claudio Luchinat, University of Florence, Florence, Italy.,

    Claudio Luchinat, Giacomo Parigi & Enrico Ravera

Authors
  1. Claudio Luchinat
    View author publications

    Search author on:PubMed Google Scholar

  2. Giacomo Parigi
    View author publications

    Search author on:PubMed Google Scholar

  3. Enrico Ravera
    View author publications

    Search author on:PubMed Google Scholar

Corresponding author

Correspondence to Claudio Luchinat.

Ethics declarations

Competing interests

The authors declare no competing financial interests.

Rights and permissions

Reprints and permissions

About this article

Cite this article

Luchinat, C., Parigi, G. & Ravera, E. The competitive world of RAS biology. Nat Chem Biol 10, 173–174 (2014). https://doi.org/10.1038/nchembio.1461

Download citation

  • Published:

  • Issue date:

  • DOI: https://doi.org/10.1038/nchembio.1461

This article is cited by

Search

Advanced search

Quick links

Nature Briefing

Sign up for the Nature Briefing newsletter — what matters in science, free to your inbox daily.

Get the most important science stories of the day, free in your inbox. Sign up for Nature Briefing