Figure 3: The GCase active site.
From: Structure of acid β-glucosidase with pharmacological chaperone provides insight into Gaucher disease
(a) Ball-and-stick representation of glycerol-bound acidic active site. (b) Ball-and-stick representation of active site seen in inh-like conformation in neutral. (c) Ball-and-stick representation of active site seen in acidic-like conformation in neutral. (d) Ball-and-stick representation of IFG-bound inh active site. Difference (Fo − Fc) electron density (green) is contoured at 3σ and was calculated using only respective GCase coordinates. Asn396 and Trp381 are omitted for clarity. Hydrogen bonding interactions to ordered water molecules are indicated by gray dashed lines. (e) Schematic diagram of hydrogen bonding interactions involved in stabilizing IFG in the active site of GCase. Distances are in Å.
