Figure 7: Flash photolysis of caged GABA discloses that rMS affects predominantly dendritic inhibition.
From: Repetitive magnetic stimulation induces plasticity of inhibitory synapses
(a) Flash photolysis of RuBi-GABA was employed to separately assess dendritic and somatic inhibition in the same set of patched CA1 pyramidal neurons (filled with Alexa568 before recordings; la-mol). Scale bar, 30 μm. (b) Representative averaged traces (five consecutive laser-light pulses at 0.1 Hz in each region) showing RuBi-GABA-uncaging-evoked somatic and dendritic responses recorded from non-stimulated control cultures and cultures between 2 and 4 h after rMS. (c,d) Group data of uncaging-evoked inhibitory postsynaptic current (uIPSC) amplitudes (n=9 neurons per group; five cultures each; Mann–Whitney test). In the same set of cultures mIPSC recordings confirmed a significant reduction in the mean mIPSC amplitude of CA1 pyramidal neurons (note: internal solution contained higher [Cl−] than in Fig. 1h; control, n=10 neurons; rMS, n=10 neurons from four cultures each; Mann–Whitney test). Individual data points are indicated by grey dots. Values represent mean±s.e.m. (**P<0.01; ***P<0.001; ns, not significant differences).
