Figure 8: TMS induces changes in gephyrin clusters in vivo.
From: Repetitive magnetic stimulation induces plasticity of inhibitory synapses
(a–c) Examples of horizontal brain slices containing the hippocampus stained for gephyrin from non-stimulated anaesthetized 3-month-old male mice and 2 h after 10-Hz rTMS. Experimental conditions were adapted to the in vitro experiments. An increase in gephyrin immunostaining co-localizing with TO-PRO nuclear stain (blue) was noted. Scale bar in a, 300 μm, in b, 50 μm, in c, 4 μm. (d,e) A significant reduction in gephyrin cluster sizes and numbers was observed in rad but not in pyr 2 h after stimulation (control, n=12 hippocampi from six animals; rTMS, n=14 hippocampi from seven animals; Mann–Whitney test). Individual data points are indicated by grey dots. Values represent mean±s.e.m. (*P<0.05; **P<0.01; ns, not significant differences).
