Figure 2: TyrRS extracellular staining and haemolymph secretion. | Nature Communications

Figure 2: TyrRS extracellular staining and haemolymph secretion.

From: Active JNK-dependent secretion of Drosophila Tyrosyl-tRNA synthetase by loser cells recruits haemocytes during cell competition

Figure 2

(a–d) Extracellular staining of endogenous TyrRS (red) in clones of wt loser cells (green, GFP) under cell competition in wing imaginal discs (a,b) or without cell competition (c,d). (e,f) Z-section of wing imaginal discs with loser cells (GFP) under cell competition, endogenous TyrRS extracellular staining (red) in clones under cell competition in wing imaginal discs (e) or with Kish RNAi co-expression (f). (g) Western blot of Lipophorin (loading control) and endogenous TyrRS in haemolymph of larvae with or without cell competition (48 ACI) and quantification of three independent experiments normalized by loading control (Lipophorin). The difference is statistically significant, T-test P<0.05. (h–j) Extracellular staining of secreted TyrRS (red, anti-HA staining) in wt loser cells (green, CD8-GFP) expressing UAS-TyrRS-HA. Extracellular secretion of UAS-TyrRS-HA is lost when Kish RNAi is co-expressed (k–m). Scale bar, 10 μm.

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