Figure 2: syb:GRASP labelling in the mushroom body calyx. | Nature Communications

Figure 2: syb:GRASP labelling in the mushroom body calyx.

From: Dynamic labelling of neural connections in multiple colours by trans-synaptic fluorescence complementation

Figure 2

(a) Experimental set-up: to visualize the syb:GRASP-labelled synapses of individual neurons, the driver R68D02-LexA was used to express syb:spGFP1–10 in two to three olfactory PNs (also labelled by tdTomato, red), while CD4:spGFP11 was targeted to Kenyon cells of the mushroom body (Kc, blue; by MB247-Gal4). Olfactory PNs synapse onto Kenyon cell dendrites in the mushroom body calyx, and then continue to the lateral horn (LH) where they also form synapses. (b–d) Robust syb:GRASP fluorescence is detected by live two-photon microscopy in the mushroom body calyx (asterisks). Some non-synaptic GRASP is also observed along the axon (arrowhead), and at least one potential synaptic contact is not labelled by GRASP fluorescence (open circle). (e–g) As expected, no syb:GRASP signal is observed in the LH, the secondary site of PN synapses, since spGFP11 is not expressed in the LH neurons. (b,e) Composite image. (c,f) Green channel, GRASP. (d,g) Magenta channel, tdTomato. Scale bar, 5 μm. Full genotype: Aop-tdTomato/+; Aop-syb-spGFP1–10, UAS-CD4:spGFP11/R68D02-LexA; MB247-Gal4/+.

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