Figure 5: NaV1.9-V1184A channels cause hyperexcitability and reduced cold-sensitivity of DRG neurons.
From: Cold-aggravated pain in humans caused by a hyperactive NaV1.9 channel mutant
(a) Representative trains of action potentials at 30 °C, recorded from murine DRG neurons transfected with wild-type NaV1.9 (black) or mutant NaV1.9-V1184A (red), in response to 2 s current injections of 60 pA. Dotted lines mark 0 mV; dashed lines indicate the RMP. (b) Action potential frequencies obtained from experiments as shown in a as a function of the injected current. (c,d) Equivalent set of experiments as shown in a and b, performed at 20 °C. Data points represent mean values obtained from four (30 °C) or five (20 °C) animals with numbers of experimental replicates indicated in parentheses. Significance between pairs of data was tested with a two-sided Student’s t-test. ***P<0.001, **P<0.01, *P<0.05.
