Figure 6: NaV1.9-V1184A and NaV1.9-L811P result in distinguishable firing characteristics of DRG neurons.
From: Cold-aggravated pain in humans caused by a hyperactive NaV1.9 channel mutant
(a) Representative trains of action potentials at 30 °C, recorded from murine DRG neurons transfected with NaV1.9 (black), NaV1.9-V1184A (red) or NaV1.9-L811P (blue), in response to 30 s current injections of 40 pA. Dotted lines mark 0 mV; dashed lines indicate the RMP. (b) Trains of action potentials as shown in a, obtained from repetitively firing neurons, were analysed to determine the fraction of neurons that maintained stable action potential amplitudes over 30 s. The criterion for ‘persistent firing’ was defined as a reduction of peak action potential amplitude (Vmax) by <10 mV throughout the train. Numbers of experimental replicates are given in parentheses. Significance was tested with a Z-test. **P<0.01.
