Figure 3: Compartment-dependent modulation of intracellular calcium dynamics in glucose-stimulated β-cells by fatty acids.

(a) Exemplary calcium traces after uncaging compounds 5 and 6, respectively; left: initiated and transiently interrupted Ca²⁺ oscillations; right: potentiated and terminated Ca²⁺ oscillations. (b) Number of detected high-intensity calcium events within every 60 s interval before and after uncaging compounds 5 and 6. (c) Distribution of high-intensity calcium events (⩾60% of highest event in each trace) in ∼390 cells over time before and after AA uncaging from compounds 5 or 6. Note: a detailed discussion of scope and limitations of the data analysis approach may be found in the Supplementary Fig. 10. P-values were obtained by Welch two sample t-test. (d) Schematic representation of cellular localization of compounds 5 and 6 before uncaging. (e) Classification of cellular responses after uncaging AA. (f) Number of detected high-intensity calcium events within every 60 s interval before and after uncaging oleic acid (OA) from compounds 9 and 10. (g) Distribution of high-intensity calcium events (⩾60% of highest event in each trace) in 304 cells before and after uncaging 9 and 10. Error bars represent s.e.m. throughout the figure.