Figure 1: Genitalia rotation requires Myo-II. | Nature Communications

Figure 1: Genitalia rotation requires Myo-II.

From: Left–right asymmetric cell intercalation drives directional collective cell movement in epithelial morphogenesis

Figure 1

(a) Adult male (left) and pupae (right). Schematic of the adult male abdomen (middle), showing the looping of the ejaculatory duct (ed) around the hindgut (hg) resulting from a 360° clockwise rotation. sp, sperm pump. (b) Schematic of male genitalia at 24 h APF. The posterior part of the A8 tergite (magenta) surrounds the innermost parts (grey), including the genitalia (A9) and analia (A10). The anterior part of A8 (green: ventral part, light blue: dorsal part) is surrounded by A7 epidermis (mauve). (c) Time-lapse series of genitalia rotation. Ventral is at the top. Green: nuclei in anterior A8 (A8a) cells, visualized by enhanced cyan fluorescent protein (ECFP) expressed by the AbdB-GAL4LDN driver. Magenta: all nuclei, visualized by His2Av::mRFP. Yellow (or black in c’) arrows indicate the direction from the analia to the external genitalia. c is schematically drawn in c’. Blue and red arrows indicate the movement of A8p and A8a, respectively. Scale bar, 100 μm. (d–f) The external genitalia pointed to the ventral side in all control flies (d), whereas most of the flies expressing sqh (e) dsRNA and zip (f) dsRNA with the AbdB-GAL4LDN driver showed orientation defect. White arrows indicate the direction from the analia to the external genitalia. Rose diagrams indicate the frequency of the external genitalia position in adult male flies. (g–i) Time-lapse series of genitalia rotation in control (g), sqh dsRNA (h) and zip dsRNA-expressing flies (i). Green: nuclei in A8a, visualized by RedStinger with the AbdB-GAL4LDN driver. Magenta: emc::GFP, which labels almost all nuclei. Yellow arrows indicate the direction from the analia to the external genitalia. Ventral is at the top. Scale bar, 100 μm. Fly genotypes: His2Av::mRFP/+; AbdB-GAL4 UAS-H2B::ECFP/+ (c), UAS-Dcr2/+; AbdB-GAL4 UAS-RedStinger/+ (d), UAS-Dcr2/+; AbdB-GAL4 UAS-RedStinger/UAS-sqh dsRNA (e), UAS-Dcr2/+; AbdB-GAL4 UAS-RedStinger/UAS-zip dsRNA (f), UAS-Dcr2/+; emc::GFP AbdB-GAL4 UAS-RedStinger/+ (g), UAS-Dcr2/+; emc::GFP AbdB-GAL4 UAS-RedStinger/UAS-sqh dsRNA (h), UAS-Dcr2/+; emc::GFP AbdB-GAL4 UAS-RedStinger/UAS-zip dsRNA (i).

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