Figure 2: The autonomous epithelial movement is involved in genitalia rotation. | Nature Communications

Figure 2: The autonomous epithelial movement is involved in genitalia rotation.

From: Left–right asymmetric cell intercalation drives directional collective cell movement in epithelial morphogenesis

Figure 2

(a) Schematic of the ex vivo culture experiment. The excised caudal region of pupae (a-1) was cleaned of internal tissues by pipetting with S2 medium (a-2), then placed into a culture tray with S2 medium. Time-lapse images were captured by a microscope. (b) Genitalia rotation occurred in the ex vivo-cultured genital disc (upper). The rotation was impaired by Y-27632 treatment (lower). Green: nuclei in the posterior of each segment, visualized by ECFP in the nucleus driven by en-GAL4. Magenta: His2Av::mRFP. Scale bar, 100 μm. (c) Graph showing the angular velocity of the ex vivo-cultured genital disc rotation, calculated for 2 h after the beginning of ex vivo culture. Error bars indicate s.d. Fly genotypes: en-GAL4 UAS-H2B::ECFP/+; His2Av::mRFP/+ (a,b).

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