Figure 2: Infection of spontaneously induced nodule primordia in double mutants. (a–f) symrk-3 snf1 nodules.

(a) Uninfected primordium. (b, c) Examples of bacteria in infection threads approaching a spontaneous nodule primordium. (d) Old, fully infected nodule. (e, f) Close-up of partly misguided infection threads with swellings. (g) Infection thread in nup85-1 snf1 root hair. (h) Fully infected nup85-1 snf1 nodule. (i) Infection thread in nup133-3 snf1 root hair above spontaneous nodule primordium. (j) Fully infected nup133-3 snf1 nodule. (k) Infection thread in L. japonicus wild-type root hair above nodule primordium. (l) Young, fully infected wild-type nodule. Infection threads and infected tissue were visualized by staining of M.loti bacteria expressing lacZ (a–f and i–l) or by fluorescence of M. loti bacteria expressing eGFP (g–h). Scale bars (a–g, i, k, l): 20 μm, (j): 50 μm, (h): 100 μm.