Figure 4: Raman images of a mouse brain slice.

(a,b) The intensity distribution of Raman peaks at 1,682 cm⁻¹ (red) and 2,848 cm⁻¹ (green) imaged by the LI (a) and SLI (b) microscopes (Scale bar, 5 μm). Raman peaks at 1,682 cm⁻¹ and 2,848 cm⁻¹ can be assigned to amide-I and CH₂ stretching vibrational modes, respectively, predominantly observed in protein beta sheets and lipids. (c) Raman spectra measured in b. (d) The line profiles of three major Raman peaks given in the CH stretching region. The intensity distributions between the two arrows in a and b are shown.