Figure 6: Caffeine stimulates adducin phosphorylation through DARPP-32. | Nature Communications

Figure 6: Caffeine stimulates adducin phosphorylation through DARPP-32.

From: DARPP-32 interaction with adducin may mediate rapid environmental effects on striatal neurons

Figure 6

(a) Striatal samples were prepared from C57BL/6 mice 40 min after intraperitoneal injection of saline or 7.5 mg kg−1 caffeine. pThr75 and total DARPP-32, and actin as loading control, were analysed by immunoblotting. Lower panel, independent data points are plotted and means±s.e.m. are shown; n=9 per group from 2 experiments; Student’s t-test, t16=2.53, *P<0.05. (b) Samples obtained as in a were immunoblotted for pSer713 and total β-adducin and actin. Lower panel as in a, n=7 per group from 2 experiments; Student’s t-test, t14=3.66, **P<0.01. (c) T75A knock-in mutant mice or WT littermates were injected with saline or caffeine as in a and killed 40 min later. Striatal samples were analysed as in b. Lower panel as in a, n=12–15 per group from >3 experiments; two-way ANOVA: caffeine factor, F(1,60)=6.28, P=0.015; genotype factor, F(1,60)=0.001, not significant (NS); no interaction, F(1,60)=2.48. Šidák post hoc test, caffeine versus saline: *P=0.01.

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