Figure 1: ER stress triggers expression and nuclear localization of proteins involved in LTC4 biosynthesis. | Nature Communications

Figure 1: ER stress triggers expression and nuclear localization of proteins involved in LTC4 biosynthesis.

From: Leukotriene C4 is the major trigger of stress-induced oxidative DNA damage

Figure 1

(a) Immunoblot of proteins expressed in WISH cells at different times after induction of ER stress with BfA. Blots are representatives of three replicates. (b–e) Immunostain of the indicated proteins following treatment of WISH cells with vehicle or BfA. Trans. is transmission light microscopy. Nuclei were counterstained with Hoechst 33258 (Hoechst). Shown are merges of 5-LO and MGST2; 5-LO, Hoechst and cPLA2; Hoechst, 5-LO and FLAP; and MGST2 and the ER marker protein disulfide isomerase (PDI). Bars, 5 μm. (f) Quantification of per cent co-localization of FLAP and MGST2 with 5-LO, as determined by analysis of confocal microscopy images. n=6, P<0.0001 for both pairs. (g) Quantification of per cent nuclear localization of the indicated proteins as determined by analysis of confocal microscopy of the images shown in panels b–e. n≥6, P<0.0001 for all samples. Values in f and g represent the mean±s.d. Statistical significance was determined using one-way analysis of variance (ANOVA).

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