Figure 2: ER stress triggers MGST2-based biosynthesis of LTC4.
From: Leukotriene C4 is the major trigger of stress-induced oxidative DNA damage

(a,b) Immunostain of LTC4 after treatment of WISH cells with vehicle or BfA. R.F. is relative fluorescence intensity. Bar, 20 μm. n=3, ***P<0.001. (c) Immunoblot of MGST2 in WISH cell extracts following transfection with siControl or siMgst2. (d,e) Immunostain of LTC4 after transfection of WISH cells with control siRNA or Mgst2 siRNA, followed by vehicle or BfA. Bar, 100 μm. n=3, ***P<0.001. Values in b and e represent the mean±s.d. Statistical significance was determined using one-way ANOVA.