Figure 3: ER stress triggers expression and nuclear localization of the LTC4 receptors.
From: Leukotriene C4 is the major trigger of stress-induced oxidative DNA damage

(a) Immunoblot of the two LTC4 receptors, CysLTR1 and CysLTR2, expressed in WISH cells at different times after induction of ER stress with BfA. Blots are representatives of three replicates. (b) Immunostain of CysLTR1 and CysLTR2 after treatment with vehicle or BfA. Merge of PDI with CysLTR1 or with CysLTR2 is shown. Bar, 5 μm. (c) Quantification of per cent nuclear localization of the LTC4 receptors as determined by analysis of the confocal microscopy images shown in panel b. n≥6 for all samples, P<0.0001 for CysLTR2 and P<0.02 for CysLTR1. Values represent the mean±s.d. Statistical significance was determined using one-way ANOVA. (d) Scheme representing the translocation events triggered by ER stress, which initiate LTC4 biosynthesis and binding to its internalized receptors.