Figure 6: Density for RNA-1 in the CPMV-B structure.
From: Mechanisms of assembly and genome packaging in an RNA virus revealed by high-resolution cryo-EM
(a) A 40-Å thick central slab through the unsharpened CPMV-B map (at 3.63 Å resolution; unsharpened map is also included in the deposition for EMDB-3013, suggested contour level 0.015). The extra density ascribed to RNA is pink. Viral coat proteins coloured as described previously. (b) The strongest density for RNA is found beneath the capsid twofold axis, a binding site formed at the interface between two adjacent pentons. (c) Close up of viral RNA–protein interactions, demonstrating two major bridges of density between the viral RNA and the protein capsid. The density bridges are consistent with the involvement of W190 and R17 (both from the L subunit) in RNA binding.
