Table 2 Refinement and model statistics for atomic models built de novo into eVLP and CPMV-B EM density.
From: Mechanisms of assembly and genome packaging in an RNA virus revealed by high-resolution cryo-EM
eVLP | CPMV-B | |
|---|---|---|
Data collection | ||
Particles | 4,998 | 4,331 |
Pixel size (Å per pixel) | 1.04 | 1.04 |
Defocus range (μm) | 0.5–5.0 | 0.5–8.0 |
Voltage (kV) | 300 | 300 |
Dose (e- per Å2) | 45 | 45 |
EM refinement | ||
Final resolution (Å) | 3.04 | 3.44 |
Experimental B-factor (Å2) | −74.6 | −107.6 |
EMDB accession number | EMD-3014 | EMD-3013 |
r.m.s. deviations | ||
Bonds (Å) | 0.0077 | 0.0108 |
Angles (°) | 1.2957 | 1.5319 |
Model validation | ||
R factor | 0.2207 | 0.2909 |
Molprobity score | 1.61 (100th percentile) | 1.76 (100th percentile) |
Clashscore (all atoms) | 0.81 (100th percentile) | 1.52 (100th percentile) |
Good rotamers (%) | 96.3% | 96.5% |
PDB ID | 5a33 | 5a32 |
