Figure 2: Interneuronal DISC1 knockdown enhances ErbB4 activation and signalling in interneurons in vivo. | Nature Communications

Figure 2: Interneuronal DISC1 knockdown enhances ErbB4 activation and signalling in interneurons in vivo.

From: Interneuronal DISC1 regulates NRG1-ErbB4 signalling and excitatory–inhibitory synapse formation in the mature cortex

Figure 2

(a) Western blot for ErbB4 and pErbB4 in cortical lysates from adult Disc1-LI mice or WT littermates. Disc1-LI mice showed greater pErbB4 signal. (b) Immunostaining for pErbB4 in cortical sections (layer 3, PFC) from adult Disc1-LI mice or WT littermates. Disc1-LI mice showed greater pErbB4 immunoreactivity in PV-positive cells (arrowheads). Scale bar, 10 μm. (c) Immunostaining for DISC1 in cortical sections (layer 3, PFC) from PV-Cre mice injected with AAV expressing Cre-dependent shRNA (Control (Cont) or DISC1) and GFP. Images from two independent and representative fields are shown. DISC1 expression is knocked down exclusively by DISC1 shRNA, specifically in GFP-positive cells (arrowheads). Scale bar, 10 μm. (d) Immunostaining for pErbB4 in PV-Cre mice injected with AAV expressing Cre-dependent shRNA (Cont or DISC1) and GFP. pErbB4 is increased in GFP-positive cells expressing DISC1 shRNA (arrowheads). Scale bar, 10 μm. (e) Immunostaining for pErk in PV-Cre mice injected with AAV-expressing Cre-dependent shRNA (Cont or DISC1) and GFP. pErk is increased in GFP-positive cells expressing DISC1 shRNA (arrowheads). Blue signal in images represents 4,6-diamidino-2-phenylindole (DAPI) stain. N>30 cells from five mice. Data are represented as mean±s.e.m. *P<0.05, **P<0.01; scale bar, 10 μm.

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