Figure 6: Interneuronal DISC1 knockdown increases inhibitory synapse formation on pyramidal neurons. | Nature Communications

Figure 6: Interneuronal DISC1 knockdown increases inhibitory synapse formation on pyramidal neurons.

From: Interneuronal DISC1 regulates NRG1-ErbB4 signalling and excitatory–inhibitory synapse formation in the mature cortex

Figure 6

(a) Western blot for GAD65 in synaptosomal fractions from adult Disc1-LI mice or WT littermates. Disc1-LI mice showed greater levels of synaptosomal GAD65. Synaptophysin was used as a loading control (Cont). (b) Immunohistochemistry showing lentiviral- and AAV-mediated cell type-specific fluorescent labelling. Pyramidal neurons and interneurons are individually labelled by RFP and GFP, respectively. (c) Immunohistochemistry for GFP (blue), GAD65 (green) and RFP (red) in PV-Cre mice injected with AAV. The majority (76%, N=13 cells) of GAD65-positive (GAD65+) boutons were also GFP-positive (GFP+), that is, originating from infected PV interneurons (arrowheads). Scale bar, 5 μm. (d) Top, immunohistochemistry for RFP (red, pyramidal neurons) and GAD65 (green) in PV-Cre mice with interneuronal shRNA expression (Cont or DISC1). GAD65+ punctae adjacent to RFP-labelled cell bodies, that is, perisomatic GAD65 boutons, were analysed. Bottom, quantification of GAD65 bouton number and size (area). DISC1 knockdown led to denser and larger perisomatic GAD65 boutons. Blue signal in images represents 4,6-diamidino-2-phenylindole (DAPI) stain (nucleus). N=14 cells from 4 mice. Data are represented as mean±s.e.m. *P<0.05, **P<0.01; scale bar, 20 μm.

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