Figure 4: A gradient of CED-3 caspase activity exists in the NSMnb. | Nature Communications

Figure 4: A gradient of CED-3 caspase activity exists in the NSMnb.

From: Engulfment pathways promote programmed cell death by enhancing the unequal segregation of apoptotic potential

Figure 4

(a) Maximum intensity single channel and overlay projections of confocal images of representative NSMnb at metaphase in wild type (+/+), ces-2(bc213), ced-3(n717), ced-1(e1735), ced-6(n1813) and ced-2(n1994) embryos expressing Ppie-1gfp::tac-1 (bcIs104) and Ppie-1mCherry::plcδph (ltIs44) (scale bar, 5 μm). Orange arrowheads indicate the PCM in the ventral part of the NSMnb and blue arrowheads indicate the PCM in the dorsal part of the NSMnb. (b) Ratios of GFP::TAC-1 pixels associated with the ventral and dorsal PCM in individual NSMnb cells at metaphase in various genetic backgrounds (wild type (+/+), ced-3(n717), ced-3(n718), ced-3(n2433), ces-2(bc213), dnj-11(bc212), ces-1(n703gf), ceh-20(ay9), ceh-30(n3714), egl-1(n1084n3082), ced-9(n1950), ced-4(n1162), ced-1(e1735), ced-2(n1994), ced-6(n1813) and ced-8(n1891)) (n=8–19). The mean ratio is given (top). For comparison, the dotted line indicates mean ratio of wild type (ratio of 1.3). Mean ratios were analysed using the Student’s t-test (**P≤0.01, ***P≤0.001 and ****P≤0.0001). All statistical analyses were done in comparisons to wild type.

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