Figure 3: BPTF is required for MYC-induced chromatin remodelling.
From: BPTF is required for c-MYC transcriptional activity and in vivo tumorigenesis
(a) DNase I hypersensitivity (DHS) at MYC-bound regions in control and BPTF-silenced HFF MYC-ER cells, determined by enzyme titration. Dots represent the average values of seven independent experiments. P values were determined using paired t-test. (b) ChIP analysis of Pan AcH3, H3K4me3 and total H3 levels at the promoter of ‘target’ and ‘non-target’ genes in control and BPTF-silenced HFF MYC-ER cells. Each gene was assayed in at least three independent experiments. ChIP values are expressed as % of input and normalized for total histone H3 (with the exception of total H3). P values were determined using paired t-test. *P<0.05; **P<0.01; ***P<0.001.
