Figure 1: GtrB catalyses the formation of UndP-glucose in vivo and in vitro. | Nature Communications

Figure 1: GtrB catalyses the formation of UndP-glucose in vivo and in vitro.

From: Structure of the polyisoprenyl-phosphate glycosyltransferase GtrB and insights into the mechanism of catalysis

Figure 1

(a) GtrB (TM domain in blue, GT domain in orange) catalyses the transfer of a glucose from UDP-glucose to UndP, producing UndP-glucose. (b) Cells overexpressing GtrB accumulate a species detected by negative ion ESI/MS at m/z of 1,007.75, corresponding to the [M-H]-ion of UndP-glucose. Its identity was further confirmed by tandem mass spectrometry (MS/MS). (c) When incubated with varying concentrations of UndP for 1 h, membranes prepared from GtrB-overexpressing cells accumulate UndP-glucose (UndP-Glc) in a concentration-dependent manner (circles), whereas those prepared from control cells overexpressing a membrane protein of unrelated function do not (squares). Error bars are provided as ±s.e.m., n=3.

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