Figure 3: Fluidic property of supramolecular nanofibre 1 evaluated by FRAP experiment and electrically regulated transport of ionic fluorescent lipids along the fibre. | Nature Communications

Figure 3: Fluidic property of supramolecular nanofibre 1 evaluated by FRAP experiment and electrically regulated transport of ionic fluorescent lipids along the fibre.

From: Fluidic supramolecular nano- and microfibres as molecular rails for regulated movement of nanosubstances

Figure 3

(a,b) Time-lapse CLSM images for nanofibres 1/3 (a) and 2/3 (b) after photobleaching. (c) Plots of fluorescence intensity depending on time for nanofibres 1/3 at marked regions ROI (region of interest) 1, 2 in the inset of c. (1=0.25 wt%, 2=0.25 wt%, FITC-lipid 3=3.0 μM in 50 mM HEPES (4-(2-hydroxyethyl)-1-piperazineethanesulfonic acid) buffer (pH 7.2)). (d) CLSM images of coil-shaped nanofibres 1/3+4 during the sequentially applied electric voltage. (e) Plots of fluorescence intensity depending on time at marked ROI 1, 2 shown in the image of d (merge, t=390 s) (1=0.25 wt%, agarose=2.0 wt%, FITC-lipid 3=3.0 μM, Cy5-lipid 4=6.0 μM in 50 mM HEPES buffer (pH 7.2); applied voltage, 15 V cm−1). Dotted lines denote times when the voltage direction was switched or voltage was cutoff. The scale bars in a, b and d are 10, 50 and 10 μm, respectively.

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