Figure 4: Regulated motions of nanobeads on supramolecular nanofibres 1/5.

(a) Time-lapse CLSM images for nanofibres 1/5 binding FITC-labelled SA after photobleaching. (b) Fluorescence recovery curve with a one-dimensional diffusion model fit (Supplementary Fig. S4) (1=0.10 wt%, biotin-lipid 5=24 μM (1.6 mol% against 1), FITC-labelled SA=18 μg ml⁻¹). (c) Time-lapse CLSM images (DIC mode) of nanofibres 1/5 attaching 250-nm SA beads. The trace merged in the right panel denotes the directed motion of the SA beads along the long axis of the nanofibres (Supplementary Movie 1). (d) Time courses of the accumulated migration distances of 250 nm SA beads at 25 °C corresponding to c. (e) Histograms of average lateral diffusion speeds of 50 nm (red, 20 individual beads), 250 nm (green, 22 individual beads) and 500 nm (blue, 19 individual beads) SA beads at 25 °C evaluated from the series of time-lapse CLSM images (Supplementary Fig. S8). (f) Averaged lateral diffusion speeds depending on the diameter of SA beads. (g) CLSM images (DIC mode) of nanofibres 1/5 attaching 250 nm SA beads in a microchannel fabricated by PDMS (50 μm in height, 300 μm in width). The trace merged in the right panel denotes the directed motion of 250 nm SA beads along the nanofibres (Supplementary Movie 2) (1=0.10 wt%, biotin-lipid 5=24 μM (1.6 mol% against 1)). The scale bars in a, c and g are 5 μm.