Figure 2: Specific ligation of NCRs leads to differential IS formation. | Nature Communications

Figure 2: Specific ligation of NCRs leads to differential IS formation.

From: Natural cytotoxicity receptor splice variants orchestrate the distinct functions of human natural killer cell subtypes

Figure 2

(a–d) Representative images of IS formation on specific engagement of NCRs of dNK or pNK cells cultured overnight with IL15 stimulated on glass coverslip surfaces coated with anti-NKp30 (a), anti-NKp44 (b), anti-NKp46 (c) and anti-NKG2A (d) antibodies. Representative images of NK cells and antibody-coated glass coverslips were acquired with a Zeiss LSM710 confocal microscope using a × 63 oil objective (Carl Zeiss AG, Jena, Germany). Polarization was imaged after 20 min of stimulation. F-actin (phalloidin in green), microtubules (α-tubulin in blue) and lytic granules containing perforin (red) were stained. Scale bar, 20 μm. Images were processed using ImageJ software. Bar graphs (right panel) represent the percentage of conjugates showing polarized perforin after receptor engagement. At least 300 conjugates were analysed. Results are given as average mean values±s.e.m. from five independent experiments. **P<0.01; NS, not significant; Student’s t-test.

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