Figure 6: Effect of GATAD1 overexpression on gene co-expression networks.

(a–c) Inferred co-expression network between the top 20 genes losing edges (green) and the top 20 genes gaining edges/connections (red) during prepubertal development, based on sum of changes in connectivity between PND14–PND21, PND14–PND28 and PND21–PND28. (a) Network at PND14. (b) Network at PND21. (c) Network at PND28. (d) Network at PND 28 with the top 20 genes losing edges (yellow) and gaining edges (blue) under GATAD1 overexpression as compared with the network inferred at PND28. In this network, genes that are among the top 20 genes gaining edges during normal prepubertal development are shown as triangles, while genes that are among the top 20 losers of connectivity during development are shown as squares. For all networks positive correlation edges are represented by solid black lines, negative correlation edges are shown as dashed lines. (e) Changes in connectivity of genes belonging to selected transcription-related functional categories that lose (Gene Ontology category GO:0045892: negative regulation of transcription, DNA-templated) or gain (Protein Information Resource, http://pir.georgetown.edu/; Keyword ‘transcription regulation’) connectivity (green and red bars, respectively) from PND14 to PND28. For each gene the alteration in connectivity resulting from GATAD1 overexpresssion as compared with PND28 is shown as blue bars. (f) Genes encoding transcriptional repressors (annotated by ‘SP_PIR_KEYWORD’ ‘transcription regulation’) belong to the group of top 20 gainers of connectivity under GATAD1 overexpression (blue bars). Most of these genes (except Setdb1) showed decreased connectivity during normal prepubertal development (green bars).