Figure 3: Stereological estimates reveal decreased cell number in Shh-depleted genitalia.
From: Sonic hedgehog controls growth of external genitalia by regulating cell cycle kinetics
(a–c) Genital tubercles of ShhcreERT2/C and control Shh+/c embryos are shown at E12.5. Pregnant females were injected with tamoxifen at E10.5 and with BrdU 44 h later. (a) Lateral view of E12.5 ShhGFPcre;R26R embryo stained with X-Gal to show Shh-expressing cells10. Red box marks region of genital tubercle (gt) and underlying cloacal endoderm shown in (b) and (c). (b, c) Control Shh+/C (b) and ShhcreERT2/C (c) embryos at ×10 magnification showing BrdU-labelled cells (red). (d–f) Estimates of volume (d) total mesenchymal cell number (e) and cell density (f) in ShhcreERT2/C (white bars; n=3) and control Shh+/c (black bars; n=3) embryos. Data represented as means with ± s.e.m. *P<0.05.
