Figure 3: 1,25D3-mediated VDR recruitment to the Cyp11a1 promoter region is altered in CD8⁺ T cells differentiated in IL-2 or IL-2+IL-4 in the presence or absence of 100 nM or 1 μM 1,25D3.

(a) Localization of VDR-binding sites and qPCR primers in the Cyp11a1 promoter region. (b) qPCR was performed using five Cyp11a1 promoter-specific primers covering seven VDR-binding sites. Data were analysed via the percent input methodology: (2^{CT of total input−CT of specific IP}) × 100 and relative percent input ratios using CD8⁺ T cells stimulated with IL-2 as baseline. Data (relative fold change+s.e.m.) are from three independent experiments. Linear mixed models were employed; pairwise comparisons were performed using t-tests derived from these models. *P<0.05, **P<0.01, ***P<0.001 compared with the IL-2 group, these P values remained significant after correction for multiple comparisons; (Benjamini–Hochberg⁵⁸ correction). No statistical significance was detected compared with the IL-2+IL-4 group.