Figure 4: Adoptive transfer of 1,25D3-treated CD8⁺ T cells into CD8-deficient recipients fails to induce AHR.

Recipient mice were sensitized (two intraperitoneal, i.p.) and challenged (four nebulizations, Neb) using secondary allergen challenge model and received no cells, CD8⁺ T cells differentiated in IL-2 alone (CD8) or in the presence of 100 nM (CD8+100 nM 1,25D3) or 1 μM 1,25D3 (CD8+1 μM 1,25D3). (a) Changes in airway resistance (RL) were measured in response to increasing concentrations of methacholine. (b) Cell composition in BAL fluid. Data (mean+s.e.m.) are from two to three experiments with three to four mice per experiment. *P<0.05, **P<0.01, ***P<0.001; compared with sensitized and challenged CD8-deficient recipients that received no cells. General linear models were employed; pairwise comparisons were performed using t-tests derived from these models. #P<0.05, ##P<0.01, ###P<0.001 compared with sensitized and challenged CD8-deficient recipients that received CD8⁺ T cells differentiated in IL-2 alone, these P values remained significant after correction for multiple comparisons (Benjamini–Hochberg⁵⁸ correction); P values that did not reach the threshold P value after adjustment for multiple comparisons are shown numerically.