Figure 2: Sox21a is sufficient to drive differentiation.
From: Accumulation of differentiating intestinal stem cell progenies drives tumorigenesis
(a,b) AMG of flies overexpressing GFP (control, a) or Sox21a (b) in progenitor cells using esgTS driver for 2 days at 29 °C. esg>GFP channel is also shown. Insets show a high-magnification view. Pdm1 staining is shown in red. (c) Quantification of the number of progenitor cells in a 10,000-μm2 area in AMG of control and overexpressing Sox21a for 3 and 6 days (n=12, 10 and 10, respectively). (d,e) AMG of flies overexpressing GFP (control, d) or Sox21a (e) in EBs using GBETS driver for 4 days at 29 °C. GBE>GFP channel is also shown. (f) Quantification of the ratio of newly formed EC-like cells to all the GFP-positive cells in AMG of control and overexpressing Sox21a for 4 days (n=5). Error bars indicate s.e.m. (c,f). P values from Student’s t-test (*P<0.05; **P<0.01; ***P<0.001) are shown in c and f, and the results represent three independent experiments. One representative image from 12 midguts tested in one experiment, which was repeated three times, is shown in a,b,d and e.
