Figure 6: Mmp2 is required for tumour progression.
From: Accumulation of differentiating intestinal stem cell progenies drives tumorigenesis
(a,b), Expression of Mmp2 detected with an endogenous Mmp2-GFP fusion protein in the AMG of 10-day-old Sox21a/+ (control, a) and Sox21a flies (b). Arm (red, plasma membrane) and Pros (red, nuclear). Note that Mmp2 is only expressed in tracheal cells in the control (arrows in a), while it is expressed in the midgut progenitor cells (Arm positive) in Sox21a flies (arrow in b). (c–e) Confocal sections of Sox21a intestines expressing GFP (control, c), bskDN (d) or Mmp2-RNAi (e) in EB for 21 days at 29 °C. EBs are in green. (f), Tumour burden in AMG of flies with the indicated genotypes monitored at 21 days at 29 °C. Kayak (Kay) encodes the c-fos component of the JNK transcription factor AP-1. timp encodes a protein inhibitor of matrix metalloproteinase. Numbers of flies scored for each genotype (in biological triplicates) are indicated in f. P values from χ2-test (*<0.05; **<0.01; ***<0.001). Each other individual image shown in a–e represents 20 flies tested in one experiment, which was repeated three times.
