Figure 7: Tumour-induced elimination of surrounding enterocytes requires JNK activation.

(a) Two-week-old Sox21a intestine stained with the JNK activity reporter puc^E69-Gal4 UAS-GFP (in green) and the basolateral cell-polarity marker Discs large (Dlg, in red). (b–d) Confocal section of intestine with EB-specific depletion of Sox21a for 2 weeks, stained with the JNK activity reporter puc^E69-lacZ (red, nuclear) and Dlg (red, plasma membrane). EBs (GBE>GFP) are in green. (c,d) High-magnification view of two tumours defined in b. (e) Tumour burden in AMG of flies with the indicated genotypes monitored at 21 days. Flies were kept at 25 °C (for mutants) or 29 °C (for misexpression). Numbers of flies scored for each genotype (in biological triplicates) are indicated in e. P values from χ²-test (*<0.05; **<0.01; ***<0.001). Each other individual image shown in a–d represents 20 flies tested in one experiment, which was repeated three times.