Figure 4: Initial and final angle distributions and relaxation time dependence of catalytic time constants.
From: ATP hydrolysis assists phosphate release and promotes reaction ordering in F1-ATPase
(a–d) Initial- (yellow) and final-angle (grey) distributions for the four simulation cases (with 40 nm bead) in Fig. 3d–g. (e) Typical initial- and final-angle distributions from one catalytic dwell of a single F1 (with 40 nm bead). Solid line: angle distribution of the catalytic dwell. (f) Ratios of the final- and initial-angle MAD from experimental data with 40 nm bead (circles) and simulations (crosses). Numbering of crosses: (1) Scheme 3; (2) Scheme 1 with slow synthesis; (3) Scheme 1; (4) Scheme 2; (5) Scheme 2 with slow synthesis; and (6) Scheme 4. The corresponding initial- and final-angle distributions are also indicated. Error bars represent the 68% confidence intervals of the MAD ratio. (g,h) Catalytic time constants versus relaxation time of angular fluctuations from experiments and simulations. Smaller time constant (g) and for the Larger one (h). Error bars: uncertainties in the estimated relaxation times and time constants fitted from rotary traces. Each point in f–h corresponds to one catalytic dwell from a single F1.
