Table 1 Identification of single-cell clones bearing NanoLuc integrations.
From: A generic strategy for CRISPR-Cas9-mediated gene tagging
Gene | gRNA ID | gRNA sequence | Tagged clones/total clones |
|---|---|---|---|
ID1 | 2655 | 5′-GTGCTGAGCGGAGCCCGGAC-3′ | 2/24 |
ID1 | 2656 | 5′-GGCGCTGATCTCGCCGTTGA-3′ | 5/24 |
IRF9 | 2659 | 5′-GGCCTTTGCCCGATACTTGC-3′ | 1/24 |
IRF9 | 2660 | 5′-AGTCTGCTCCAGCAAGTATC-3′ | 0/24 |
TAP2 | 2663 | 5′-TGCGGGACAGAAACAACGTC-3′ | 0/24 |
TAP2 | 2664 | 5′-CATCCAGGATGAGGACCCGC-3′ | 0/24 |
CCL2 | 2665 | 5′-ACAGATCTCCTTGGCCACAA-3′ | 0/24 |
CCL2 | 2666 | 5′-TCAGGATTCCATGGACCACC-3′ | 1/24 |
IL6 | 669 | 5′-GTGCCTGCAGCTTCGTCAGC-3′ | 3/24 |
