Table 3 Cells bearing TurboGFP-tagged alleles can be enriched by FACS sorting.

From: A generic strategy for CRISPR-Cas9-mediated gene tagging

Gene

GFP positive (pre-sorting)

GFP positive (post-sorting)

LMNA

1.1%

75.9%

TERF1

0.4%

21.5%

LAMP1

0.4%

26.3%

  1. FACS, fluorescence-activated cell sorting; GFP, green fluorescent protein; gRNA, guide RNA.
  2. HAP1 cells containing TurboGFP-tagged LMNA, TERF1 or LAMP1 were analysed for GFP positivity by FACS, either before or after FACS sorting.