Figure 3: Characterization of CXCR4+ cells. | Nature Communications

Figure 3: Characterization of CXCR4+ cells.

From: Genetically engineering self-organization of human pluripotent stem cells into a liver bud-like tissue using Gata6

Figure 3

(a) GATA6-expressing cells upregulate expression of CXCR4 (left). CXCR4-expressing cells surround TRA-1-81 compact cellular clusters at day 5 (right). TRA-1-81 recognizes hiPSC compact clusters at undifferentiated state. Scale bars, 200 μm. (b) Single-cell analysis of Dox-induced cells (1,000 ng ml−1) at day 5 shows ∼93% of CXCR4-expressing cells are also GATA6+. (c) CellTrace fluorescent stain labelled hiPSCs on day 0 before Dox (P0). The cells are transduced with Dox-inducible GATA6-2A-EGFP vector. (d) After 4 days of Dox (1,000 ng ml−1) treatment, GATA6-induced cells express EGFP (P1), proliferate and dilute the fluorescent dye through subsequent divisions to levels comparable to GATA6 non-expressing cells (P2) (e) CXCR4+ transcriptional profiling, D, Day of the experiment (D0=Addition of Dox to the cell culture medium). Log2 NI, log 2 normalized intensities. Scale bars, 200 μm.

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