Figure 8: Maintenance of the central position of the metaphase plate does not rely on tension but could depend on a viscous cytoplasm.
From: F-actin mechanics control spindle centring in the mouse zygote
(a) Embryos expressing His–RFP alone (left panels) or together with cVCA (right panels). Upper panel: cVCA (red), one Z-plane is shown; middle and lower panels: His–RFP, purple or black, Z-projection over 20 μm. One frame is shown every 30 min. Scale bar, 10 μm. (b) Graph showing the position of the centroid of the chromosomes in the referential of the embryo (0 being the centre) from metaphase to anaphase for representative embryos expressing cVCA. The scale is represented by the white box in Fig. 8a. Each colour represents an embryo. The arrows point to the last position recorded before anaphase. One time point is shown every 20 or 30 min.
