Figure 5: Reduced liver triglyceride (TG) by free Raptor is PHLPP2-dependent.
From: mTORC1-independent Raptor prevents hepatic steatosis by stabilizing PHLPP2
(a–d) Western blots from liver (a), hepatic TG (b), plasma TG (c) and liver lipogenic gene expression (d) of adult Ad-GFP and Ad-Raptor mice co-transduced with control (Ad-shControl), Ad-shPHLPP1 or Ad-shPHLPP2 adenoviruses, killed after a 16 h fast followed by 4 h refeeding (n=6 per group). (e) Hepatic TG from liver of adult Ad-GFP and Ad-Raptor mice co-transduced with control (GFP) or myrAkt adenovirus, killed after a 16 h fast followed by 4 h refeeding (n=6 per group). (f) GTT in adult, HFD-fed Ad-GFP or Ad-PHLPP2 mice (n=6–7 per group). *P<0.05, **P<0.01 as compared with the indicated control by two-way analysis of variance. All data are shown as the means±s.e.m. All blots are representative of three independent experiments, and samples within each group chosen randomly.
