Figure 3: Label-free prediction of DNA content and cell cycle phases for fixed Jurkat cells treated with a prophase blocking agent.
From: Label-free cell cycle analysis for high-throughput imaging flow cytometry
(a) Based only on brightfield and darkfield features, we find a Pearson’s correlation of r=0.894±0.032 (error bars indicate the s.d. obtained via 10-fold cross-validation) between actual DNA content and predicted DNA content using regression (see Methods section). We applied the Watson pragmatic algorithm to determine the G1, S and G2/M phases in the DNA histograms. (b–d) For cells that are actually in a particular phase (for example, b shows cells in G1/S/G2), the bar plots show the classification results (see Methods section) (for example, b shows that the few cells in prophase (Pro) and the other mitotic phases (others) are errors). Note that we grouped the cells in metaphase, anaphase and telophase into one class since we only detected very little cells in those phases after treatment with the prophase blocking agent. (e) Bar plot of the true positive rates of the cell cycle classification. Using boosting with random undersampling to compensate for class imbalances, we obtain true positive rates of 65.5±6.3% (P), 85.8±1.4% (G1/S/G2) and 100% (others).
